Journal: Arteriosclerosis, thrombosis, and vascular biology

Article Title: Single-Cell Multimodal Profiling Highlights Persistent Aortic Smooth Muscle Cell Changes in Diabetic Mice Despite Glycemic Control

doi: 10.1161/ATVBAHA.125.324012

Figure Lengend Snippet: A. UMAP clustering of combined Xenium data sets from diabetic db/db and diabetic mice treated with DAPA for 6 weeks (n=4). B. Dot plot showing the cell specific markers from combined analysis of Xenium data sets. C. UMAP plot showing clustering of different cell types in aorta from db/db and db/dbDAPA mice. D. Representative Xenium data showing distribution of cell specific markers of SMC ( Myh11 ), fibroblast ( Dcn ) and endothelial cells ( Pecam1 ) in a representative aorta from one of the db/dbDAPA mice. E. Aortic cell clusters are depicted clearly in aortic sections in Xenium data sets from db/db and db/dbDAPA group. F-H. Representative images of Xenium data from db/db and db/dbDAPA aortas showing the expression of contractile ( Myh11 ) and Fibrosis markers ( Fn1 and Col1a1 ). I-L. Violin plots depicting expression of contractile ( Myh11 , Acta2 ), and fibrosis associated genes ( Fn1, Col1a1 ) in db/db and db/dbDAPA groups (showing DAPA reduces Col1a1 fibrosis marker).

Article Snippet: For fibronectin 1 (FN1) IHC, the sections were incubated with anti-FN1 rabbit polyclonal primary antibody (1:300, cat# 15613–1-AP, Proteintech), followed by goat anti-rabbit secondary antibody (1:200, cat# BA-1000, Invitrogen).

Techniques: Spatial Transcriptomics, Expressing, Marker